模式生物果蝇在水环境污染物毒理学中的应用及研究进展

经济发展以及人类活动加速造成生态环境破坏,工业及生活污水的过度排放使得江河、海水的污染加剧,对人类健康的危害也日益加深。果蝇作为一种经典的模式生物,与其它动物模型相比,具有饲养成本低、生命周期短、繁殖能力强、遗传物质简单、突变表型多且易于观察等诸多优点,是一种应用广泛并适合于研究人类疾病和毒理学的模式生物。简述了水环境中挥发性有机化合物、氟喹诺酮类药物、重金属、农药和微生物毒素等污染物对果蝇的毒理学研究,提出了果蝇作为经典的模式生物在研究毒理学机制以及筛选抗毒药物的优势,并对未来的发展趋势做了展望。     

Exploiting chemical ecology to manage hyperparasitoids in biological control of arthropod pests

Insect hyperparasitoids are fourth trophic level organisms that commonly occur in terrestrial food webs, yet they are relatively understudied. These top‐carnivores can disrupt biological pest control by suppressing the populations of their parasitoid hosts, leading to pest outbreaks, especially in confined environments such as greenhouses where augmentative biological control is used. There is no effective eco‐friendly strategy that can be used to control hyperparasitoids. Recent advances in the chemical ecology of hyperparasitoid foraging behavior have opened opportunities for manipulating these top‐carnivores in such a way that biological pest control becomes more efficient. We propose various infochemical‐based strategies to manage hyperparasitoids. We suggest that a push‐pull strategy could be a promising approach to ‘push’ hyperparasitoids away from their parasitoid hosts and ‘pull’ them into traps. Additionally, we discuss how infochemicals can be used to develop innovative tools improving biological pest control (i) to restrict accessibility of resources (e.g. sugars and alternative hosts) to primary parasitoid only or (ii) to monitor hyperparasitoid presence in the crop for early detection. We also identify important missing information in order to control hyperparasitoids and outline what research is needed to reach this goal. Testing the efficacy of synthetic infochemicals in confined environments is a crucial step towards the implementation of chemical ecology‐based approaches targeting hyperparasitoids. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

渤海湾外来浮游植物物种及其入侵途径分析

为了预防外来浮游植物入侵引发的渤海湾赤潮灾害，基于近20年建设项目环境影响评价报告书和相关文献资料的研究成果，收集整理了渤海湾海域已发现的外来浮游植物物种及具体物种信息，分析了物种入侵的主要途径及可能产生的赤潮灾害影响。结果表明：渤海湾海域共发现17种外来浮游植物，其中，甲藻门最多，有10种；硅藻门次之，有5种；金藻门和黄藻门最少，各有1种。研究表明：渤海湾外来浮游植物中，除中华盒形藻外，其余16种均属于赤潮生物，这些物种主要通过压载水引入、传播的方式进入渤海湾海域，应加强港口监控和管理，以防赤潮灾害。     

菜豆细菌性萎蔫病菌传入中国的风险分析

从世界分布、主要寄主、经济重要性、传入可能性和风险管理难度5个方面对菜豆细菌性萎蔫病菌传入我国的风险进行评估。结果表明,该病菌是特别危险的有害生物。根据分析结果提出了非疫区输入、输出国应严格检疫、进境口岸加严检查等相应的风险管理措施。     

利用QuantStudioTM 3D数字PCR分析转基因玉米MON863含量

QuantStudioTM 3D数字PCR (QuantStudioTM 3D digital PCR,3D-dPCR)是一种基于超高密度亲疏水微孔芯片实现数字PCR分液原理的新型核酸绝对定量平台,在转基因生物定量领域具有极大的应用前景.本研究基于3D-dPCR平台,以转基因玉米(Zea mays)MON863混合样品为例,建立基于单重和双重数字PCR体系的转基因生物(genetically modified organisms,GMOs)含量分析方法.与传统qRT-PCR比较发现,在缺乏样品纯度、纯合度信息的情况下,数字PCR能够较好地排除这些因素的影响,测定准确的量值.研究结果表明,QuantStudioTM 3D数字PCR是一种适用于转基因生物含量分析的精确定量方法,还可反映转基因玉米种子的基因型.本研究基于3D-dPCR建立的转基因玉米MON863单重和双重定量方法为转基因检测提供了新的方法和参考.     

Diversity of Glomale Mycorrhizal Fungi in Maize/Sesbania Intercrops and Maize Monocrop Systems in Southern Malawi

The study investigated diversity and frequency of occurrence of glomale (Arbuscular- or Vesicular Arbuscular-) mycorrhizal fungi on three farming systems in a drought prone and nitrogen deficiency site in southern Malawi. The farming systems comprised of two agroforestry systems of Sesbania sesban (L) Merr intercropped with maize and Sesbania macrantha E Phillips & Hutch. intercropped with maize and a maize monocrop systems without fertilizer, with nitrogen, phosphorus and a combination of nitrogen and phosphorus. Species diversity and species frequency of occurrence were examined in soil samples obtained in the dry and wet seasons. Twelve glomale mycorrhizal species were recorded, four species being in the genus Acaulospora, four in Glomus, two in Gigaspora and two in Scutellospora. Species diversity in the two agroforestry systems were not significantly (p ≤ 0.05) different but had lower species diversity than maize monocrop with only Sesbania macrantha intercropped with maize significantly (p ≤ 0.05) lower. Species diversity was significantly increased by the inorganic nitrogen fertilizer. Inorganic phosphorus fertilizer had no effect. The study shows that the occurrence and persistence of glomale species are influenced by agroforestry combinations, and that the spores of most species are tolerant to dry conditions. Only four species responded to fertilizer application with the occurrence of spores of some species high and some low. Management practices have great implication in the persistence of spore propagules of glomale species. The order Glomale was revised in 2001 and upgraded to a phylum Glomeromycota.     

大豆深加工产品定性PCR检测影响因素分析

以大豆内源基因（Lectin）、筛选基因35S启动子（Cauliflower mosaic virus 5S，CaMV35S）、NOS终止子（Nopaline synthase，Nos）和外源基因（CP4 EPSPS）为检测对象，对大豆深加工产品的DNA提取方法、PCR扩增条件的退火温度、引物浓度进行探讨，得出不同DNA提取方法、退火温度、引物浓度对大豆加工产品PCR检测的影响，建立了大豆加工食品中转基因成分定性检测体系。检测结果表明，退火温度60℃、引物浓度0．4μM时所建立的定性PCR检测方法能有效检测出大豆加工产品中的转基因成分，而且方法简单、快速有效。     

港口疏浚淤泥悬浮物对2种海洋生物的急性毒性试验研究

从上个世纪开始,人们对于海洋悬浮物就予以了高度重视。悬浮物作为海洋中最普遍的污染,对海洋生物造成了很大危害。本文用海洋疏浚淤泥悬浮物对卤虫无节幼体和梭鱼幼鱼进行了急性毒性试验。结果显示,半致死浓度随着时间的延长而逐渐降低;不同密度的海洋生物半致死浓度相差不大。不同水温条件下的梭鱼幼鱼试验表明,水温为24℃时其半致死浓度比21℃时要低,其安全浓度也呈现相同的趋势。     

我国林业外来有害生物的入侵与防范措施

主要是对当前我国外来有害生物的入侵并蔓延原因进行分析,并提出对外来有害生物的防范措施。     

河北黄骅沿海海洋生物体中重金属残留量及评价

主要研究了河北省黄骅沿海海洋生物体内铅、匐和铬等重金属含量。分析结果表明，贝类体内含量高于钎类及早壳类，但其含量均低于评价标准，铅具有较明显季节变化。单因子评价结果，美质量分的指数均小于１，表明该海域海洋生物受重金属污染水明显。